Quartz crystal microbalance based nanosensor for lysozyme detection with lysozyme imprinted nanoparticles.

نویسندگان

  • Gulsu Sener
  • Erdogan Ozgur
  • Erkut Yılmaz
  • Lokman Uzun
  • Ridvan Say
  • Adil Denizli
چکیده

The aim of this study is to prepare quartz crystal microbalance (QCM) nanosensor for the real-time detection of lysozyme. In the first part, the lysozyme imprinted (MIP) nanoparticles were prepared by mini-emulsion polymerization. The MIP nanoparticles were characterized by TEM, zeta-sizer and FTIR-ATR measurements. Particle size was found around 50 nm. The MIP nanoparticles were attached by dropping of nanoparticle solution to gold surface and then, dried at 37°C for 6h. QCM nanosensor was characterized with AFM and ellipsometer. The observations indicated that the nanoparticle film was almost monolayer. The detection limit was found as 1.2 ng/mL. The specificity of the QCM nanosensor was shown by using albumin as a competitor molecule. The results show that the QCM nanosensor has high selectivity and sensitivity with a wide range of lysozyme concentrations in both aqueous solutions (0.2-1500 μg/mL) and natural sources (egg white) (460-1500 ng/mL).

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عنوان ژورنال:
  • Biosensors & bioelectronics

دوره 26 2  شماره 

صفحات  -

تاریخ انتشار 2010